Normal mouse lung fibroblasts (MLg, ATCC catalog n°CCL-206) were cultured in minimal essential medium (MEM, Invitrogen) supplemented with 10% fetal bovine serum (FBS, Invitrogen) and 1% of antibiotic-antimycotic (AA, Invitrogen) at 37 °C with 5% CO2. The cells were distributed in 96-well culture plates (Cellstar, Ref 655180, Greiner) at a density of 20,000 cells per well in complete medium (10% FBS, 1% AA). THP-1 monocytes (ATCC catalog n°TIB-202) were cultured in RPMI-1640 (Invitrogen) supplemented with 10% FBS, 1% AA, 1 mM sodium pyruvate (Invitrogen) and 10 mM HEPES (Invitrogen) at 37 °C with 5% CO2. The cells were distributed in a 96-well culture plate at a density of 100,000 cells per well in complete medium (10% FBS, 1% AA, 1 mM sodium pyruvate, 10 mM HEPES) and differentiated into macrophages by addition of 100 nM phorbol 12-myristate 13-acetate (PMA, Sigma Aldrich). The cells were cultured at 37 °C for 24 h before MWCNT exposure.

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