WT and Tg21 in the postnatal age ranges of P13–P15 and P19–P22 were briefly anaesthetized with isoflurane and decapitated. The number of recordings for each group are as follows; at P13–P15, WT n(cells) = 8, n(animals) = 4 and for Tg21 n(cells) = 9, n(animals) = 5; at P19–P22, WT n(cells) = 8, n(animals) = 6 and for Tg21 n(cells) = 8, n(animals) = 6. The brain was quickly removed and transferred to ice-cold dissection solution containing 65 mm NaCl, 2.5 mm KCl, 1.25 mm NaH2PO4, 25 mm NaHCO3, 7 mm MgCl2, 0.5 mm CaCl2, 25 mm glucose and 105 mm sucrose, saturated with 95% O2 and 5% CO2. 350 μm-thick sagittal slices containing the hippocampus were cut from the tissue block with a vibratome (Leica) and kept in oxygenated ACSF (315 mOsm) containing 125 mm NaCl, 2.5 mm KCl, 1.25 mm NaH2PO4, 25 mm NaHCO3, 1 mm MgCl2, 2 mm CaCl2, and 25 mm glucose at 32°C for 25 min and then at RT until use.

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