E. coli strains from glycerol stocks were grown in LB at 37 °C at 150 rpm overnight and then sub-cultured at 1/100 dilution in M9 medium. For growth experiments, 1 × 107/100 μl of bacterial cells were inoculated in a transparent 96 well plate (Sarstedt, Nümbrecht, Germany) and were grown with shaking at 37 °C. Bacterial growth (OD600nm) was assessed at every hour. For luminescence measurements, 1 × 107/100 μl of bacterial cells were inoculated in a white 96 well plate (Greiner Bio-One, Kremsmünster, Austria) and were grown with shaking at 37 °C. Light emission (luminescence (count/s) expressed as relative light units (RLU)) was recorded every hour in parallel with OD in a Spark® multimode microplate reader (TECAN, Québec, Canada).

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