Animals were injected with pentobarbital (100 mg/kg, i.p.) for anesthesia, and were transcardially perfused with 30 ml of 1× PBS. Following perfusion, brains were removed from the skull and cortical and hippocampal tissues were dissected on ice-cold 1× PBS for RNA analysis. For Western blot analysis, forebrain tissue samples were collected and frozen in dry ice immediately for later use. For isolation of corpus callosum, whole-brain samples were harvested and dissected with the aid of a dissecting microscope to isolate corpus callosum. During the isolation process, brains were kept in ice-cold 1× PBS. Following isolation, corpus callosum tissue samples were immediately frozen in dry ice until later use.

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