The sequences of PoCD9.1 (Gene ID:109,624,332) and PoCD9.3 (Gene ID:109,644,010) were obtained by PCR from flounder head kidney cDNA based on the sequences in the NCBI with the primers PoCD9.1F/PoCD9.1R and PoCD9.3F/PoCD9.3R (Table (Table1),1), which were designed by Primer Premier 5. The sequence was analysed using the BLAST program at the NCBI. The domain was searched using the simple modular architecture research tool. The calculated molecular mass and theoretical isoelectric point were predicted by the Compute pI/Mw tool. The cartoon diagram was manufactured by Protter. The phylogenetic tree was constructed based on the amino sequence alignment by the neighbour-joining (NJ) method with MEGA 7.0 software, and bootstrap trials were replicated 1000 times.

Primers used in this study.

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