The sequences of PoCD9.1 (Gene ID:109,624,332) and PoCD9.3 (Gene ID:109,644,010) were obtained by PCR from flounder head kidney cDNA based on the sequences in the NCBI with the primers PoCD9.1F/PoCD9.1R and PoCD9.3F/PoCD9.3R (Table (Table1),1), which were designed by Primer Premier 5. The sequence was analysed using the BLAST program at the NCBI. The domain was searched using the simple modular architecture research tool. The calculated molecular mass and theoretical isoelectric point were predicted by the Compute pI/Mw tool. The cartoon diagram was manufactured by Protter. The phylogenetic tree was constructed based on the amino sequence alignment by the neighbour-joining (NJ) method with MEGA 7.0 software, and bootstrap trials were replicated 1000 times.

Primers used in this study.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.