Sixty- to 90-d-old adult Long–Evans male (N = 17) and female (N = 20) rats were housed in a temperature and humidity-controlled animal facility on a 12/12 h dark/light reverse cycle and had ad libitum access to food and water before experimentation. All procedures were approved by the Institutional Animal Care and Use Committee (IACUC) of Arizona State University. All animals used for chemogenetic ChI manipulation were bred in-house and confirmed ChAT::cre-positive through genotyping described below. Breeder ChAT::cre-positive males (Long–Evans- Tg(ChAT-cre5)5.1 Deis) were purchased from Rat Resource and Research Center (RRRC, RRC#658) and bred in-house with Long–Evans wild-type females purchased from Envigo.

All ChAT::cre self-administration data presented here are included within a larger data set exploring the role of sex and strain on nicotine self-administration in our recently published study (Leyrer-Jackson et al., 2020). However, while these animals were included within the Long–Evans male and female groups, the study did not focus on the subset of animals as reported here, but rather included in a larger dataset focusing on self-administration parameters across strain and sex.

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