Superoxide dismutase (SOD) and catalase (CAT) were obtained from Aladdin Biochemical Technology Co. Ltd (Shanghai, China). mPEG and PLGA (Mn = 15 000) were obtained from Sigma-Aldrich Co. Ltd. All other reagents were analytical grade and were used without further purification/modification.

Specific pathogen-free male Sprague Dawley (SD) rats (180 ± 10 g), obtained from the Experimental Animal Center, Nantong University (Nantong China) were used in the experiments. Five rats were housed in each cage within a conventional animal facility (22 ± 3°C, 45–70% relative humidity; 12 h light/dark cycle) and were fed with standard pelleted food and water ad libitum. All procedures involving animals and their care were approved by the institutional committee of animal care.

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