The 143B cells were seeded in a 24-well plate at 5 × 104 cells/well. After the cells became adherent, reagents were added to each of the following groups: the control group, the PUC19 group, the CS/PUC19 group, the SPCIONPs/PUC19 group and the PEI/PUC19 group. The magnetic field was applied for 30 min. The cells were collected by centrifugation and resuspended in a mixture of 1 µl A (1 mM Live-Dye) + 1 µl B (1 mg ml−1 PI) + 1 ml staining buffer at 24 h and 48 h. After incubation at 37°C for 15 min in the dark, the cells were observed under a fluorescence microscope. The following method was used to calculate the cell survival rate: number of red cells/(number of red cells + number of green cells) ∗ 100%.

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