Prussian blue can stain iron atoms in cells. First, 143B cells were seeded in 12-well plates at 1 × 105 cells/well. After the cells became adherent, SPCIONPs/pIGFBP5 were added, and a magnetic field was applied for 30 min. After 4 h, the cells were washed three times with phosphate-buffered saline (PBS) and fixed in paraformaldehyde for 20 min. Perls solution was prepared at a 1 : 1 ratio, and 200 µl of the solution was added to each well, followed by incubation at 37°C for 40 min. Cells were washed with ddH2O, and 200 µl of neutral red solution was added and incubated with the cells at room temperature for 2 min. After the staining solution was rinsed with ddH2O, the cells were observed under a light microscope.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.