Citric acid, ferric chloride hexahydrate, ferrous sulfate heptahydrate, 25–28 wt% ammonia solution and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) were purchased from Macleans (Shanghai, China); Dulbecco's Modified Eagle Medium (DMEM) and trypsin were purchased from Gibco (CA, USA); chitosan (50 kDa, 98% polyacetyl), n-hydroxysuccinimide (NHS) and fluorescein isothiocyanate (FITC) were purchased from Sigma-Aldrich (MO, USA); fetal bovine serum was purchased from PAN-Biotech (Adenbach, Germany); the Prussian blue staining kit was purchased from Solarbio (Shanghai, China); the DNA marker was purchased from Tiangen (Beijing, China); Hoechst, DNase 1 and LB broth were purchased from Beyotime Biotech (Beijing, China); and the Endo-free Plasmid Maxi Kit-25 was purchased from OMEGA (GA, USA).

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