Recombinant caspase‐8 (residues 217–479) without the CARD domain subcloned into pET23b (Novagen) with C‐terminal His6‐affinity tags was expressed as has been described (Backus et al, 2016) previously. Site‐directed mutagenesis (Liu & Naismith, 2008) was conducted as has been described previously, using the primers shown in the Reagents and Resources Table.

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