Brain sections (20 μm) were stained with Nissl staining reagent (Beyotime, Shanghai, China) for 20–30 min and then rinsed with double-distilled water for 5 min, 70% ethanol solution for 5 s, and 95% ethanol solution for 5 s. Then, the brain sections were dehydrated in anhydrous ethanol, cleared with xylene solution (Sinopharm, Shanghai, China), and mounted with neutral gum (Yiyang, Shanghai, China) (Jyothi et al., 2015; Fathalla et al., 2017).

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