Brain sections (20 μm) were stained with Nissl staining reagent (Beyotime, Shanghai, China) for 20–30 min and then rinsed with double-distilled water for 5 min, 70% ethanol solution for 5 s, and 95% ethanol solution for 5 s. Then, the brain sections were dehydrated in anhydrous ethanol, cleared with xylene solution (Sinopharm, Shanghai, China), and mounted with neutral gum (Yiyang, Shanghai, China) (Jyothi et al., 2015; Fathalla et al., 2017).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.