Cerebrospinal fluid samples were collected using a 28G butterfly needle connected to a 1-mL syringe. After being anesthetized with chloral hydrate (10%, 10 mL/kg, i.p.) (Keshi, Chengdu, China), the surface of the mouse brain was held vertically on the work surface, and the needle was inserted vertically (relative to the work surface) between the protuberance and the spine of the atlas. After a puncturing sensation was felt, the CSF was slowly collected (Gu et al., 2012).

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