Cerebrospinal fluid samples were collected using a 28G butterfly needle connected to a 1-mL syringe. After being anesthetized with chloral hydrate (10%, 10 mL/kg, i.p.) (Keshi, Chengdu, China), the surface of the mouse brain was held vertically on the work surface, and the needle was inserted vertically (relative to the work surface) between the protuberance and the spine of the atlas. After a puncturing sensation was felt, the CSF was slowly collected (Gu et al., 2012).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.