The study was conducted in compliance with standard animal use practices and was approved by the Animal Ethics Committee of Qingdao University (QDU20180120, Jan-2018). Thirty male C57BL/6 mice were purchased from Beijing Vital River Laboratory Animal Technology Co. (Beijing, China). The mice were maintained in a facility with a 12-h light-dark cycle and were provided with food and water ad libitum. At the time of use, the mice were 7 weeks old and weighed 20–25 g.

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