NK cells were stimulated by co-culture with K562 target cells at a 1:1 ratio. CD107a degranulation was assessed at 2 h by surface staining for flow cytometry. In parallel cultures, cytokine production was measured after 4 h (with GolgiStop and GolgiPlug [BD Biosciences] added at recommended concentrations within the first hour) by fixation with Cytofix/Cytoperm reagent (BD Biosciences) and intracellular staining for cytokine production.

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