The EBV-transformed B cell line SMI-LCL was described previously.41 K562 cells expressing 4-1BBL and IL-21 (clone 46) were kindly provided by Dr. Katayoun Rezvani (MD Anderson Cancer Center, Houston, TX, USA). Both were cultured in RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum (FBS).

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