After treatment, protein lysates were collected for Western blot analysis. A total of 15 μg of protein was used for SDS-PAGE. After primary and secondary antibody incubations, the signal was detected by a chemiluminescence detection kit, imaged by Syngene (Imgen Technologies). Antibodies for PUMA (for IHC), NAG-1 (GDF15), P53 were from Santa Cruz Biotechnology; for caspase 8, cleaved caspase 8, caspase 9, caspase 3, cleavage PARP, eIF2α, p-eIF2α (Ser51), CHOP, ATF4, DR5, FOXO3a, p-FOXO3a (Ser253), NF-κB p65, p-NF-κB p65 (Ser536), c-Jun, p-c-Jun (Ser63), JNK, p-JNK (Thr183/Tyr185), PUMA (for WB), MYC, phosphor-S62-cMYC, NDRG1, Phospho-CDK9 (Thr86), CDK9, Rpb1 NTD (RNA PII subunit B1), phosphor-(Ser2) Rpb1 CTD (RNA PII subunit B1), RSK, and phospho-p90RSK(Ser380) were from Cell Signaling Technology. Noxa and p21 were from Calbiochem. p73 was from Bethyl laboratories Inc., Ran was from BD Biosciences. β-actin was from Sigma.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.