After treatment, protein lysates were collected for Western blot analysis. A total of 15 μg of protein was used for SDS-PAGE. After primary and secondary antibody incubations, the signal was detected by a chemiluminescence detection kit, imaged by Syngene (Imgen Technologies). Antibodies for PUMA (for IHC), NAG-1 (GDF15), P53 were from Santa Cruz Biotechnology; for caspase 8, cleaved caspase 8, caspase 9, caspase 3, cleavage PARP, eIF2α, p-eIF2α (Ser51), CHOP, ATF4, DR5, FOXO3a, p-FOXO3a (Ser253), NF-κB p65, p-NF-κB p65 (Ser536), c-Jun, p-c-Jun (Ser63), JNK, p-JNK (Thr183/Tyr185), PUMA (for WB), MYC, phosphor-S62-cMYC, NDRG1, Phospho-CDK9 (Thr86), CDK9, Rpb1 NTD (RNA PII subunit B1), phosphor-(Ser2) Rpb1 CTD (RNA PII subunit B1), RSK, and phospho-p90RSK(Ser380) were from Cell Signaling Technology. Noxa and p21 were from Calbiochem. p73 was from Bethyl laboratories Inc., Ran was from BD Biosciences. β-actin was from Sigma.

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