Single-cell sequencing data were analyzed using the Seurat package. The ggplot2 package was used to produce the single-cell analysis graph. Cox regression analysis was performed using the glmnet and survival packages. The nomogram model was established by the rms package. The survival curve was generated by the survival package. P < 0.05 was regarded as statistically significant. All the statistical analyses were performed by R language, version 3.6.1.

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