All the bioassays were independently replicated five or more times unless mentioned. The data obtained was statistically analyzed in SPSS software version 22 (IBM SPSS, NY, USA), and the obtained results are reported as mean ± standard error mean (SEM) of five or more replicates. One-way analysis of variance (ANOVA) was carried out by using Tukey’s test (p < 0.001). The LC50 and LC90 values with their 95% confidence limits were determined by probit analysis, which helped to analyze the dose–mortality response [9].

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