The chemical compositions of HLJDT were collected from the Traditional Chinese Medicine Systems Pharmacology database (TCMSP, http://lsp.nwu.edu.cn/) (Ru et al., 2014), which is a unique database platform of Chinese herbal medicine system pharmacology for capturing the relationship among drugs, targets, and diseases. Those meeting the criteria of bioavailability (OB) ≥30% and drug-likeness (DL) ≥0.1 (Xu et al., 2012) were used for further analysis. The HPLC chromatogram of HLJDT obtained from the methanol extract of HLJDT is shown in Supplementary Figure S1. The potential gene targets of the major compounds in HLJDT were searched from the TCMSP and PubMed databases. Totally, 448 HLJDT-related targets were obtained (Supplementary Table S2).

The target gene number (PDB ID number) was converted into a gene name corresponding to the Genecards database through the UniProt database. The key words “myocardial ischemia” or “myocardial infarction” were input into the Genecards database (http://www.genecards.org, a database providing a detailed genome, proteome, transcription, genetic, and functional overview of all known and predicted human genes) and the Online Mendelian Inheritance in Man database (OMIM) to collect target genes related to MI. The common target genes were screened out as the target of HLJDT in the treatment of MI.

In order to explain the potential role of the active components in HLJDT in gene function and signaling pathways, we used DAVID (database for Annotation, Visualization and Integrated Discovery) to analyze the KEGG pathways of the predicted targets. The results of KEGG enrichment analysis were visualized using the Omicshare online analysis platform. The components-targets-pathway (C-P-T) network of HLJDT for treating MI was constructed by utilizing the network-visualization software Cytoscapev3.6.1.

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