The GSE138709 single-cell transcriptome profiles of ICC and adjacent tissue cell samples were downloaded from the Gene Expression Omnibus (GEO) database. The adjacent tissue was regarded as normal tissue. ICC sample clinical information and gene expression data were downloaded from The Cancer Genome Atlas (TCGA) data portal and GEO database (GSE107943) in August 2020. All the data in this study were obtained directly from the public database, and the relevant guidelines were strictly followed, allowing ethical approval to be exempted. The workflow is shown in Figure 1.

Flowchart of the study. The scRNA-seq data of 33991 cell samples were downloaded, nine prognosis-related genes (PRGs) were identified and the robustness and validity verified.

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