Attractant/Repellant Bioassay
This protocol is extracted from research article:
Bioefficacy of Lemongrass and Tea Tree Essential Oils Against House Fly, Musca domestica
Proc Natl Acad Sci India Sect B Biol Sci, Feb 18, 2021; DOI: 10.1007/s40011-020-01220-z

The attractant/repellent assays were carried out by double choice method [7] where 20 individuals of newly emerged adult flies were released in a cage containing 2 conical flasks. One flask contained 0.1% test oil in 5 mL (v/v) of milk, while the other contained acetone as solvent with 5 mL of milk to serve as a control. The EO containing conical flask was fitted with a funnel (10 cm diameter) outwards to avoid the escape of the flies (Fig. 1d). The number of flies attracted toward test oils, and solvent only in control flask were recorded after 24 h of treatment, and the percent (%) repellency was determined by using the formula;

where C is the number of flies trapped in control flask, T denotes the number of flies trapped in treated flask.

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