The adulticidal assay on M. domestica was carried out according to the method described previously [7] with minor changes. The bioassays were designed such that the flies would have a maximum probability of exposure to the tested concentration of EOs. The adult houseflies (n = 10) were transferred to a plastic jar of 1.2 dm3 having a cotton yarn attached internally to the cap (Fig. 1b). The cotton yarn was sprayed with different concentrations of EO in the range of 10–100 mg/dm3 which was dissolved in 10 µL acetone. To avoid direct contact of flies with EO, the cotton yarn was covered with a thin muslin cloth. In case of control set, the cotton yarn enclosed in muslin cloth contained only 10 µL of acetone. The experiments were replicated 10 times at 28 ± 2 °C for 30 min to calculate the mortality rate caused by EO treatment.

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