Blood samples collected into 3.2% Trisodium citrate were centrifuged at 2400g for 10 min to yield plasma aliquots which were stored at −80 °C or in temperature monitored dry ice until analysis. The prothrombin time, activated partial thromboplastin time, Clauss fibrinogen and D dimer were measured using a CS-2500 series analyzer (Sysmex Corp. Kobe, Japan) using Siemens Healthcare reagents (Marburg, Germany). The Factor VIII activity was determined using a one-stage activity assay with Actin FS activator and FVIII deficient plasma from Siemens Healthcare.

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