The meta-analysis was performed by means of Review Manager 5.3 (Cochrane Collaboration, Oxford, UK). As for dichotomous variables, the ORs were calculated, reporting 95% CI. Survival outcomes were summarized by using the generic inverse variance method. Overall survival (OS) was defined as the time from diagnosis until death. Disease-free survival (DFS) was defined as the interval between the initial primary diagnosis of CRC and the first relapse or death. Hazard ratio (HR) and 95% CI were calculated to assess the association between high level of F. nucleatum and survival. A fixed model was performed in aggregating and analyzing for results when I2< 50%. If I2 >50%, the random-effects analysis was utilized. The pooled effects were determined by conducting a Z test, and the statistical significance was defined as the two-sided P < 0.05.

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