Relapse survival analyses of the hub genes were conducted with the “survival” and the “survminer” R package based on Kaplan-Meier mothed. The minimal proportion of observations per group was set to 0.25. Log-rank p-value < 0.05 was considered as significant statistically.

Expression differences of prognosis-associated genes between BRAFV600E and BRAFWT PTCs were validated in 108 patients collected from GEO using the nonparametric Wilcoxon rank-sum test. p-value < 0.05 was considered statistically significant.

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