The pGL4.26-pBIMWT, pGL4.26-pBIKWT, pGL4.26-pBAXWT, pGL4.26-pNOXAWT, and their corresponding mutant luciferase vectors were co-transfected with Renilla (internal control) into the Control-KD (knockdown), FOXO3a-KD, Control-OE (overexpression), and FOXO3a-OE cells, respectively. After an 18 h recovery at 37 °C, the cells were used for luciferase assay with a Dual Luciferase Reporter Assay System (Promega, USA, #E1910). The relative luciferase activity was normalized using firefly luciferase activity/Renilla luciferase activity.

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