The staining intensities of HPV58 E7, Ki-67 and p16 were graded on a scale from 0 to 3+ (1+: weak, 2+: moderate, and 3+: strong) 10. In brief, the positive reaction for three antibodies was scored into four grades, according to the intensity of the staining: 0, 1, 2 and 3. The percentages of positive cells were also scored into five categories: 0 (0%), 1 (1-25%), 2 (26-50%), 3 (51-75%) and 4 (76-100%). The product of the intensity by percentage scores was used as the final score, which was categorized into four groups: 0 (<1), 1+ (1-3), 2+ (3-5), 3+ (>5). The immunostained specimens were independently evaluated by two blinded investigators. There was close agreement (>90%) between the two investigators; in the case of any disagreement, final grading was determined by consensus.

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