Total cell lysates from MG63 cells were immunoprecipitated using anti-CtBP1- and IgG-coupled protein A beads (Sigma-Aldrich, #16-125), respectively. Cell lysates and beads were incubated at 4 °C overnight, followed by washing five times with RIPA buffer. The input proteins and the purified CtBP1-coupled and IgG-coupled proteins were subjected to western blotting to examine the protein levels of CtBP1, p300, and FOXO3a, respectively.

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