Total DNA was extracted from 200 µl exfoliated cervical cell suspension with the HPV DNA extraction kit (TELLGEN, Shanghai, China) according to the manufacturer's instructions. Using the HPV DNA genotyping kit (TELLGEN, Shanghai, China), the Luminex® xMAP™ technology can quickly and quantitatively detect 27 types of HPV (high risk subtypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56 , 58, 59, 66, 68, 26, 53, 82, and low risk subtypes 6, 11, 40, 42, 43, 44, 55, 61, 81, 83). Briefly, 5 µl extracted DNA solution was used for PCR in a reaction (20 µl) containing 10 µl PCR mixture, 5 µl primer mixture, and 0.8 µl rTaq polymerase. The PCR amplification was performed and 3 µl products were added to microsphere hybrid for hybridization procedure on an ABI9700 (ThermoFisher, Waltham, U.S.A.). Each reaction pore was added 75 µl SA-PE. The mixture was detected on Luminex200 multifunctional flow detector (R&D system, Minneapolis, U.S.A.).

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