The coding sequences of CtBP1 and HIPK2 genes were cloned into empty pCDNA3 vectors using the BamHI and XhoI sites. The wild type (WT) promoters (2,000 bp) of BIM, BIK, BAX, and NOXA genes were respectively cloned into empty pGL4.26 luciferase vectors using the SacI and XhoI sites. The generated pGL4.26-pBIMWT, pGL4.26-pBIKWT, pGL4.26-pBAXWT, and pGL4.26-pNOXAWT plasmids were used as templates to create their corresponding mutants in which the FOXO3a consensus site (A/G)TAAA(T/C)A was mutated to CAGGGAG. The primers used for vector constructions were included in Supplementary Table 1. All plasmids were sequenced to verify their correct constructions.

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