Circ_SMAD2 or RPN2 3'UTR with binding sites for wild-type or mutant miR-1258, were cloned into pmirGLO vector (Promega) and named WT-circ_SMAD2, MUT- circ_SMAD2, WT-RPN2 or MUT-RPN2. The corresponding luciferase reporter and miR-1258, or miR-NC, were co-transfected into the CRC cells. Luciferase activity was measured using a dual-luciferase reporter system (Gene, China).

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