The transfected cells were dissociated with trypsin and twice rinsed with PBS 1X. Cells were then fixed in 70% ethanol at 4 °C overnight. Cell cycle analyses were done using a FACS Calibur flow cytometer (BD Biosciences) after propidium iodide (PI) staining for 30 min in the presence of RNase A (KeyGEN Biotech, Nanjing, China).

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