TGF-β and SC-79 were obtained from Beyotime Co (Shanghai, China). Z-FL-COCHO (ZFL) was purchased from Calbiochem Co. (Darmstadt, Germany). Anti-E-cadherin (#5023), anti-ZO-1 (#13663), anti-Claudin 1 (#13995), anti-AKT (#4685), anti-p-AKT (#4060), anti-PI3k (#4249), anti-p-PI3k (#17366), anti-mTOR (#2972), anti-p-mTOR (#2971), and anti-β-actin (#4970) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-CTSS (ab134157), anti-N-cadherin (ab18203), anti-occludin (ab216327) antibodies were purchased from Abcam (Cambridge, MA, USA). For F-actin staining, Phalloidin-iFluor 488 (ab176753) was obtained from Abcam (Cambridge, MA, USA).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.