A t-test with a p value < 0.05 was applied to identify significance with statistical differences. The OS analysis was performed using R programming of “survival” and “survive” packages online. The OS, RFS, PFS, and DSS were separated and calculated by the median gene expression level of E2F4. Spearman's correlation was applied to identify the relationship between E2F4 and associated genes and pathways. A p value < 0.05 was used to identify significant differences.

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