The human PTC cell line BCPAP were purchased from Guangzhou Jennio Biological Technology Co., Ltd. (cell number: 273, Guangzhou, China), and the cell identity was validated by iCell Bioscience Inc. (Shanghai, China) using short tandem repeats. It was the fourth generation of cells. The other PTC cell line TPC-1 were provided by School of public health, Shandong First Medical University & Shandong Academy of Medical Sciences. BCPAP cells were cultured and passaged in medium containing 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA) and TPC-1 cells were cultured and passaged in medium containing 10%FBS (BI, North America, USA), 1% penicillin/streptomycin solution (Beyotime Biotechnology Co., Ltd., Shanghai, China), and 89% RPMI 1640 (Corning Inc., Corning, NY, USA) in 37 °C incubators with 5% CO2. The human normal thyroid cell line HTori-3 was purchased from BeNa Culture Collection (cell number: 338687, Beijing, China) and cultured and passaged in medium containing 10% FBS (ExCell, South America) and 90% F-12K (Gibco) in 37 °C incubators with 5% CO2.

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