The human PTC cell line BCPAP were purchased from Guangzhou Jennio Biological Technology Co., Ltd. (cell number: 273, Guangzhou, China), and the cell identity was validated by iCell Bioscience Inc. (Shanghai, China) using short tandem repeats. It was the fourth generation of cells. The other PTC cell line TPC-1 were provided by School of public health, Shandong First Medical University & Shandong Academy of Medical Sciences. BCPAP cells were cultured and passaged in medium containing 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA) and TPC-1 cells were cultured and passaged in medium containing 10%FBS (BI, North America, USA), 1% penicillin/streptomycin solution (Beyotime Biotechnology Co., Ltd., Shanghai, China), and 89% RPMI 1640 (Corning Inc., Corning, NY, USA) in 37 °C incubators with 5% CO2. The human normal thyroid cell line HTori-3 was purchased from BeNa Culture Collection (cell number: 338687, Beijing, China) and cultured and passaged in medium containing 10% FBS (ExCell, South America) and 90% F-12K (Gibco) in 37 °C incubators with 5% CO2.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.