MS/MS spectra were searched using MASCOT engine (Matrix Science, London, UK; version 2.2) against a non-redundant International Protein Index arabidopsis sequence database v3.85 from the European Bioinformatics Institute ( For protein identification, the options were as followed: Peptide mass tolerance=20 ppm, MS/MS tolerance=0.1 Da, Enzyme=Trypsin, Missed cleavage=2, Fixed modification: iTRAQ 8 plex (K), iTRAQ 8 plex (N-term), Variable modification: Oxidation (M), Decoy database pattern=Reverse. The MASCOT search results for each SCX elution were further processed using the Proteomics Tools (version 3.05) 19.

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