Human prostatic cancer cell line DU145 and PC-3, and human prostatic stromal cell line WPMY-1 were obtained from Chinese Academy of Sciences and cultured in RPMI-1640 or DMEM medium with 10% fetal bovine serum (Gibco, USA) at 37°C in a humidified incubator with 5% CO2. DTX was obtained from Jiangsu Hengrui Medicine Co, Ltd.

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