Conventional and germ-free Ross PM3 broilers were hatched in the Infectiology of Farm, Model and Wildlife Animals Facility (PFIE, Centre INRAE Val De Loire: https://doi.org/10.15454/1.5572352821559333E12; member of the National Infrastructure EMERG’IN) as described by (Guitton et al., 2020). Briefly, Ross PM3 eggs from two French farms were collected, decontaminated with a 1.5% peracetic acid solution (1.5% Divosan Plus VT53, Johnson Diversey, France). Eggs were then incubated, decontaminated a second time as described above and placed in a hatching incubator for production of conventional chicks or an isolator for the production of germ-free animals. Bacteriological controls were performed as described by (Guitton et al., 2020); animals were confirmed to be bacteria-free while conventional chickens developed a microbiota.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.