To investigate the functions of VrCOL2, a 35S: CDS-VrCOL2 plasmid was constructed. The VrCOL2 CDS was amplified from the cDNA of the sequenced mungbean variety VC1973A using primers with XhoI and XbaI digestion site sequences. The resulting PCR fragment was digested by the restriction endonucleases XhoI and XbaI to generate sticky ends. The pPTN1171 vector was digested with XhoI and XbaI to generate a linearized plasmid (Ping et al., 2014). Then the VrCOL2 and pPTN1171 fragments were ligated using T4 DNA ligase (Promega). The constructed plasmid was verified by sequencing. It was then introduced into Arabidopsis using the floral dip method (Bent, 2006), and successful transformation was confirmed by PCR. All primers are listed in Supplementary Table 1.

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