To investigate the functions of VrCOL2, a 35S: CDS-VrCOL2 plasmid was constructed. The VrCOL2 CDS was amplified from the cDNA of the sequenced mungbean variety VC1973A using primers with XhoI and XbaI digestion site sequences. The resulting PCR fragment was digested by the restriction endonucleases XhoI and XbaI to generate sticky ends. The pPTN1171 vector was digested with XhoI and XbaI to generate a linearized plasmid (Ping et al., 2014). Then the VrCOL2 and pPTN1171 fragments were ligated using T4 DNA ligase (Promega). The constructed plasmid was verified by sequencing. It was then introduced into Arabidopsis using the floral dip method (Bent, 2006), and successful transformation was confirmed by PCR. All primers are listed in Supplementary Table 1.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.