Animal studies were carried out at animal laboratory center of South China Agricultural University and approved by the Animal Research Committee of South China Agricultural University (2018030). All animal studies were performed with specific-pathogen-free female KM mice (Southern Medical University, Guangdong, China), 6–8-weeks old, weighing 20 ± 2 g.

A mouse septicemia protection assay was used to assess in vivo treatment efficacy of IBG (Thangamani et al., 2016). KM female mice were treated intraperitoneally with a dose of 40 mg/kg IBG. After 24 h, KM female mice were infected with 0.1 ml of bacterial suspension (MRSA YXMC004P) via tail vein injection, a concentration that achieves about 90% mortality within 18 days after infection. At 0.5 h and 24 h post infection, mice (18 per group) were treated with 40 mg/kg IBG, 15 mg/kg VAN and PBS via intraperitoneal injection. Mice were monitored for 18 days after MRSA infection and the statistical analysis was performed by non-parametric log-rank test.

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