The MIC and MBC of IBG were determined by broth microdilution according to CLSI guidelines (Clinical and Laboratory Standards Institute, 2018). The MBC was defined as the lowest concentration where a 99.9% colony count reduction was observed (Mohammad et al., 2015). The presence of fetal bovine serum (Tianhang Biotechnology, Zhejiang, China) on IBG activity against S. aureus was also tested. Experiments were performed in triplicates.

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