MEFs were cultured in media as previously described. Ad5CMVCre and Ad5CMVeGFP (University of Iowa Viral Vector Core Facility, Iowa City, IA, USA) were thawed and added to MEF media along with polybrene (Santa Cruz Biotechnology); after 24 hours, the MEFs were washed with 1x Dulbecco's phosphate-buffered saline (DPBS) (Thermo Fisher Scientific) and fresh media was added. GFP fluorescence was visualized using a fluorescence microscope.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.