MEFs were cultured in media as previously described. Ad5CMVCre and Ad5CMVeGFP (University of Iowa Viral Vector Core Facility, Iowa City, IA, USA) were thawed and added to MEF media along with polybrene (Santa Cruz Biotechnology); after 24 hours, the MEFs were washed with 1x Dulbecco's phosphate-buffered saline (DPBS) (Thermo Fisher Scientific) and fresh media was added. GFP fluorescence was visualized using a fluorescence microscope.

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