The histological visualization of fat cells and neutral fat was observed by Oil red O staining (Sigma-Aldrich) during CCl4 treatment with or without CQ. In brief, approximately 5×5×3-mm cuboids from the right lobe of the liver were fixed with 4% paraformaldehyde, cryoprotected with 30% sucrose, and then embedded with OCT compound (Sakura Finetek, Torrance, CA, USA). Then, 5-μm frozen slices were stained with Oil Red O for 15 min at room temperature. Afterwards, the dye was discarded and counterstained with hematoxylin. After pouring out the dye solution and using water to wash it until blue, glycerin-gelatin was used as a seal and observed under a microscope.

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