2.2.1. Measurements of sleepiness, alertness, sleep latency and sleep length

Sleep diaries and triaxial wrist accelerometers (ActiSleep, ActiGraph, Maribo Medico A/S, Maribo, Denmark) were used to find time in bed and total sleep time for the two nights of habitual sleep and sleep restriction. The Karolinska sleepiness scale (KSS) (Akerstedt and Gillberg, 1990) were used to calculate subjective sleepiness. Before pain testing began, a psychomotor vigilance test (PVT) (Basner and Dinges, 2011) (custom written C++ program, National Institute of Occupational Health, Norway) lasting 10 min, was used to assess behavioral alertness by measuring reaction time. After the other tests were completed, EEG + electrooculography (EOG) was used to assess sleep latency. The lighting was reduced to below 1 lux and the subject was asked to try to fall asleep, while sitting in a reclined chair for 20 min.

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