Cut-off values of NLR and PLR were calculated using maximum point (sensitivity + specificity) of the Receiver Operating Characteristic (ROC) curve for the prediction of distant metastasis. The area under curves (AUCs) were 0.688 (0.648–0.728) and 0.638 (0.598–0.678) for NLR and PLR, respectively. Using this method, cut-off values for NLR and PLR were 2.8 and 170, respectively (Supplementary Figure 1).

The associations of NLR, PLR, distant metastasis and other clinicopathological variables were evaluated using Chi-square or Fisher's exact tests. Univariate and multivariate tests were performed using a logistic regression model. Odds ratio (ORs) and the corresponding 95% confidence intervals (95% CIs) were reported with P value < 0.05 considered as statistically significant. All statistical tests were performed using SPSS 19.0 software (SPSS Inc., Chicago, Ill).

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