HPLC system (Shimadzu, Japan) is equipped with a binary gradient system with a variable UV/VIS detector (SPD-20A) and a Rheodyne injector with a 20 μL loop and LC-20AD pumps and integrator. Reversed-phase chromatographic analysis was performed in isocratic conditions using a C18 reverse-phase column (Super coil LC-18; 25 cm × 4.6 mm ID; 5μm particle size; 100A° Pore size; Phase-ODS) at 37 °C.

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