The plasma sample was added to phenol in 50 mM sodium phosphate buffer (pH 7.4) mediated nitration of phenol after incubation for 2 h at 37 °C and NaOH was added and read absorbance at 412 nm. The yield of nitrophenol was calculated from 4400 M−1 cm−1 as an index of peroxynitrite concentrations.

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