A549 cells were fixed and incubated with primary antibodies. The primary antibodies used in this study were rabbit polyclonal antibody against ACE2 for immunofluorescence (Sino Biological, 10108-T26) and anti-influenza virus-NP antibody (kindly provided by Professor Ningshao Xia). Alexa Fluor dye-conjugated secondary antibodies (Alexa Fluor R488, Invitrogen; Alexa Fluor M555, Invitrogen) and DAPI (Beyotime, C1002) were administered afterward according to standard protocols. Cell imaging was performed on a Leica TCS SP8 confocal laser scanning microscope (Leica).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.