A549 cells were fixed and incubated with primary antibodies. The primary antibodies used in this study were rabbit polyclonal antibody against ACE2 for immunofluorescence (Sino Biological, 10108-T26) and anti-influenza virus-NP antibody (kindly provided by Professor Ningshao Xia). Alexa Fluor dye-conjugated secondary antibodies (Alexa Fluor R488, Invitrogen; Alexa Fluor M555, Invitrogen) and DAPI (Beyotime, C1002) were administered afterward according to standard protocols. Cell imaging was performed on a Leica TCS SP8 confocal laser scanning microscope (Leica).

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