For western blot analysis, cells were lysed in RIPA buffer on ice for 30 min, separated via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and subjected to western blot analysis. For mouse experiments, half of the lung tissue from each mouse was homogenized in PBS, followed by boiling in SDS lysis buffer (GE) at 100 °C for 30 min. Rabbit monoclonal antibody against ACE2 (Abclonal, A4612, 1:1000), mouse monoclonal antibody against SARS-CoV Nucleoprotein (Sino Biological, 40143-MM05, 1:1000), and anti-actin antibody (Abclonal, 1:1000) were purchased commercially. The anti-influenza virus-NP antibody was kindly provided by Professor Ningshao Xia. Peroxidase-conjugated secondary antibodies (Antgene, 1: 5000) were applied accordingly, followed by image development with a Chemiluminescent HRP Substrate Kit (Millipore Corporation).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.